Journal: ESC Heart Failure

Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

doi: 10.1093/eschf/xvag229

Figure Lengend Snippet: Circulating biomarkers levels in chronic heart failure patients and controls

Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

Techniques:

Journal: ESC Heart Failure

Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

doi: 10.1093/eschf/xvag229

Figure Lengend Snippet: Circulating miRNAs and cytokines as molecular signatures of heart failure. Differences in serum levels of GDF-15 and FGF-21 in CHF patients and controls. Serum levels of GDF-15 by ELISA assay in patients with heart failure (HF) ( n = 25) and the control group ( n = 10). ( A ) Data showed significant upregulation of GDF-15 in CHF vs controls ( P < .001). ( B ) FGF-21 levels did not differ significantly between groups. Correlations between GDF-15 and selected miRNAs in CHF patients. ( C , D ) In CHF patients, GDF-15 levels were positively associated with miR-29b ( ρ = 0.42, P = .037) and miR-486 ( ρ = 0.42, P = .039). Data are expressed as fold change and are presented as mean ± SEM. Student's t -test or Mann–Whitney test was used for normally distributed data or non-normally distributed data, respectively. *** P ≤ .001.

Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control, MANN-WHITNEY

Journal: ESC Heart Failure

Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

doi: 10.1093/eschf/xvag229

Figure Lengend Snippet: Molecular profiles in CHF patients. Differences in serum levels of GDF-15 and FGF-21 among heart failure patients with and without cardiac cachexia. Circulating levels of GDF-15 and FGF-21 were analyzed in CHF patients with cardiac cachexia (CC) ( n = 10) and without cardiac cachexia (nCC) ( n = 15), and a control group ( n = 10). ( A ) Significant difference in GDF-15 levels was observed between patients with CC or nCC vs controls (all P < .001). ( B ) Levels of FGF-21 were significantly higher in CHF with CC compared with nCC ( P = .012) and vs controls ( P = .010) ( C ). Spearman’s correlation analysis revealed that in CHF patients with CC, GDF-15 negatively correlated with miR-21 ( ρ = −0.81, P = .007). ( D ) In the same group, FGF-21 negatively correlated with miR-486 ( ρ = −0.69, P = .035). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05, *** P ≤ .001.

Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

Techniques: Control, Analysis

Journal: ESC Heart Failure

Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

doi: 10.1093/eschf/xvag229

Figure Lengend Snippet: Molecular profiles in cachectic patients with severe CHF. FGF-21 concentrations and their associations with NYHA functional class in patients with CHF. ( A ) Data show higher FGF-21 levels in NYHA class III-IV ( n = 13) compared with class I-II ( n = 9) ( P = .050) and the control group ( n = 10) ( P = .050). ( B ) In NYHA class III-IV, FGF-21 positively correlated with miR-15b ( r = 0.68, P = .014). Data are expressed as fold change and are presented as mean ± SEM. One-way ANOVA test or Kruskal–Wallis test was used for normally distributed data or non-normally distributed data, respectively. * P < .05.

Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

Techniques: Functional Assay, Control

Journal: ESC Heart Failure

Article Title: Integrated miRNA-cytokine profiling reveals a molecular signature of cachexia in patients with chronic heart failure

doi: 10.1093/eschf/xvag229

Figure Lengend Snippet: Schematic representation of the circulating molecular profile associated with chronic heart failure and cardiac cachexia, characterized by dysregulation of muscle- and remodelling-related miRNAs and increased stress-responsive cytokines. CHF patients showed reduced miR-15b, miR-29b, miR-133a, and miR-486 and increased GDF-15, while cardiac cachexia was further associated with lower miR-29b and higher FGF-21 levels.

Article Snippet: Serum concentrations of GDF-15 (#ELH-GDF15-1) and FGF-21 (#ELH-FGF21-1) were measured using ELISA (RayBiotech, Norcross, GA, USA) according to manufacturers’ instructions.

Techniques: